簡(jiǎn)介:艾睿生物提供SMC-1細胞(1411D)實(shí)時(shí)價(jià)格和產(chǎn)品詳細說(shuō)明,敬請來(lái)電咨詢(xún)!詳細介紹SMC-1細胞(1411D)。
詳細說(shuō)明:
SMC-1細胞(1411D)——含 量:99 產(chǎn)品規格:100000-1000000cells/ml 產(chǎn)品包裝:液體瓶裝 品 牌:ATCC細胞庫、ScienCell實(shí)驗室等細胞提供單位 產(chǎn) 地:美國、**
細胞規格:100000-1000000cells/ml
儲存:液氮(凍存)或復蘇培養
運輸:干冰 用途:細胞領(lǐng)域科學(xué)研究
SMC-1細胞(1411D)于原代凍存或復蘇培養(這個(gè)細胞提供的周期會(huì )長(cháng)點(diǎn),少則半個(gè)月,多則一兩個(gè)月)。每管含有細胞數>5×100000cells/ml,此細胞通過(guò)**熒光染色驗證,經(jīng)測試不含有HIV-1、HBV、HCV、支原體、**、酵母和**。細胞可以達到10倍增。 公司長(cháng)期提供細胞產(chǎn)品,很多細胞科研單位都特意訂購我公司X細胞,經(jīng)常購買(mǎi)我們公司細胞產(chǎn)品的單位有:天津醫科大學(xué)、寧夏醫科大學(xué)、**軍醫大學(xué)、徐州醫學(xué)院、哈爾濱醫科大學(xué)、廣州中醫藥大學(xué)、第三軍醫大學(xué)、上海交大醫學(xué)院、蘭州大學(xué)、貴州省遵義市遵義醫學(xué)院、湖北武漢同濟醫院神經(jīng)外科、江蘇省人民醫院、北京大學(xué)人民醫院等單位的重點(diǎn)實(shí)驗室。
細胞凍存和復蘇實(shí)驗原理:細胞凍存及復蘇的基本原則是慢凍快融,實(shí)驗證明這樣可以限度的保存細胞活力。目前細胞凍存多采用甘油或二甲基亞砜作保護劑,這兩種物質(zhì)能提*細胞膜對水的通透性,加上緩慢冷凍可使細胞內的水分滲出細胞外,減少細胞內冰晶的形成,從而減少由于冰晶形成造成的細胞損傷。復蘇細胞應采用快速融化的方法,這樣可以**細胞外結晶在很短的時(shí)間內即融化,避免由于緩慢融化使水分滲入細胞內形成胞內再結晶對細胞造成損傷。
細胞凍存和復蘇實(shí)驗注意事項:
1. 從增殖期到形成致密的單層細胞以前的培養細胞都可以用于凍存,但*為對數生長(cháng)期細胞。在凍存前***換一次培養液。
2. 將凍存管放入液氮容器或從中取出時(shí),要做*防護工作,以免**。
3. 凍存和復蘇*用新配制的培養液。
人肺動(dòng)脈平滑肌細胞凍存和復蘇的原則:慢凍快融
當細胞冷到零度以下,可以產(chǎn)生以下變化:細胞器脫水,細胞中可溶性物質(zhì)濃度升*,并在細胞內形成冰晶。
如果緩慢冷凍,可使細胞逐步脫水,細胞內不致產(chǎn)生大的冰晶;相反,結晶就大,大結晶會(huì )造成細胞膜、綱胞器的損傷和破裂。復蘇過(guò)程應快融,目的是防止小冰晶形成大冰晶,即冰晶的重結晶。
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SMC-1細胞(1411D)**相關(guān)產(chǎn)品:
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